Does dahlia flower extract have anti-diabetic properties?

Does dahlia flower extract have anti-diabetic properties?

In a recent study published in Metabolism of lifeFor the first time, researchers used a diet-induced obesity (DIO) mouse model to test the effects of a yellow petal extract from… Dahlia pinata As a new treatment for type 2 diabetes (T2D).

Next, they conducted a randomized, controlled study in people with prediabetes and T2D to test dose-dependent glucose-lowering effects, safety and efficacy.

Study: Dahlia flower extract has antidiabetic properties by improving insulin function in the brain. Image credit: Shingo76Misumaru/Shutterstock.com

background

Glucose intolerance and insulin resistance (IR) are major features of T2D pathogenesis. Claude Bernard discovered that the brain controls blood glucose levels in 1885.

About 150 years later, researchers revisited his findings and found that dysregulation of central pathways of glucose homeostasis in the hypothalamus was considered the root cause of type 2 diabetes.

Specifically, circulating insulin reaches the hypothalamus, binds its receptors, and autophosphorylates it, which in turn recruits and phosphorylates insulin receptor substrate (IRS).

It activates the phosphatidylinositol 3-kinase (PI3K)-protein kinase B (AKT) pathway that initiates the cascade of metabolic effects of insulin.

Studies have shown that inflammation in the hypothalamus promotes IR via the nuclear factor kappa B kinase subunit beta (IKKβ)/nuclear factor kappa B (NF-κB) inflammatory pathway.

Thus, pharmacological inhibition of NF-κB in arcuate nucleus (ARC) neurons of the hypothalamus could attenuate glucose intolerance and help treat hypothalamic inflammation.

Preclinical studies have shown that the IKKβ inhibitor biotin significantly reduces glucose and insulin sensitivity in DIO mice by targeting hypothalamic inflammation.

Bark Toxicodendron vernisiflumThe Chinese lacca tree is considered the best natural source of butane, a rare flavonoid, although it has limited medical use due to its toxicity.

About the study

In this study, the researchers tested the effect Dr.. Pinata Flower extract, a non-toxic ornamental flower plant that contains biotin.

They obtained male C57BL/6 mice aged 12 to 14 weeks and fed them a high-fat diet (HFD) to induce obesity. Mice fed a low-fat diet (LFD) were used as controls.

After four weeks, they administered one dahlia extract and 3.3 and 10 mg/kg body weight (BW) orally to test mice. Likewise, controls received 0.9% sodium chloride (NaCl) containing 5% ethanol (EtOH). They also injected 1.5 g/kg body weight glucose intraperitoneally (i.p.).

One hour after dahlia extract administration, they performed an intraperitoneal glucose tolerance test (ipGTT) on all DIO mice. They used a glucometer to measure blood glucose levels.

The team also tested the effects of other flavonoids, such as 10 mg/kg biotin, sulforitin, isoliquiritigenin, or their combinations in another group of mice.

After administering insulin i.p., they also performed an insulin tolerance test (ITT) on a group of mice fed an HFD or LFD. In addition, the researchers conducted chronic treatment studies on mice fed an HFD or LFD My choice For five weeks.

Furthermore, the team performed intracerebroventricular injections of PI3K inhibitors in mice.

These animals then received dahlia extract (10 mg/kg body weight) via oral feeding after 1 hour, and after another 60 minutes, they were subjected to ipGTT. The researchers also performed immunohistochemistry (IHC) on coronal cryosections of the mouse brain.

Furthermore, the team studied the role of the IKKβ-NF-κB pathway using ultralight microscopy in zebrafish incubated for six hours in HFD and after five hours in dahlia extract (2.75 μg/ml).

Finally, the team conducted a clinical study among 13 males aged 18-70 years with glycated hemoglobin (HbA1c) concentrations between 40-65 mmol/mol to test the effects of three doses of dahlia extract.

They first provided all participants with capsules containing 5, 20, or 50 mg of dahlia extract powder.

Next, they subjected them to the baseline oGTT. After fasting for 12 hours overnight, they had all participants drink 75 grams of anhydrous glucose dissolved in water, drew venous blood samples, and sampled their blood every 30 minutes for up to three hours for glucose, C-peptide, and insulin. Levels of quantification.

The team compared the area under the curve (AUC) of oGTT over three hours for the three doses of dahlia extract with the area under the curve (AUC) of baseline oGTT. They monitored them for adverse reactions and tracked their complete blood counts.

results

In DIO rats, orally administered EtOH dahlia extract containing high amounts of biotin resulted in improved glucose tolerance and insulin sensitivity.

These effects were not diminished after chronic treatment, suggesting that they could help maintain glucose homeostasis in the long term. Furthermore, chronic treatment did not change the mice's liver morphology, liver fat content, or weight.

However, ICV application of leptin in DIO mice was ineffective, reflecting the effect of leptin on intestinal barrier function and differences in blood-brain barrier (BBB) ​​function between HFD-fed and leptin-deficient mice.

Chalcone isoliquiritigenin and orosulfuritin in combination with biotin also elicited glucose-lowering effects in DIO rats, likely through a functional interaction. Alive.

Further studies should be conducted to determine whether flavonoids in the extract or their metabolites mediate the beneficial effects of dahlia extract on glucose homeostasis.

At the molecular level, high-density feeding led to low-grade inflammation in the hypothalamus, which ultimately led to the development of type 2 diabetes.

It also induced reactive astrogliosis in the hypothalamus, reflecting the pro-inflammatory nature of the HF diet, which contributed to functional impairment of the neural circuits controlling energy balance.

Hypothalamic insulin signaling, especially PI3K, mediates the blood sugar regulating effects of dahlia extract.

Furthermore, dahlia extract prevented the onset of astrogliosis after HFD feeding, and improved glucose tolerance was associated with suppression of inflammatory signaling pathways within the hypothalamus.

In zebrafish, dahlia extract reduced hyperactivation of the NF-κB pathway but did not reduce NF-κB activity to levels lower than controls.

Although dahlia extract improves blood sugar regulation in people with prediabetes and T2D, in five participants with HbA1c ≥ 48 mmol/mol, the glucose-lowering effect of the 60 mg/m2 dahlia extract dose was more pronounced, suggesting that the Dahlia was effective. Most effective in treating patients who have already developed T2D.

Conclusions

In conclusion, the results of the current study showed that the extract of yellow petals of… D- Pinnate Restoration of glucose homeostasis in HFD-fed mice.

Furthermore, it was safe and effective in humans, necessitating further testing of its efficacy and safety as a treatment for people with type 2 diabetes.

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